Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1978-11-27
1980-12-09
Padgett, Benjamin R.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 424 12, G01N 3316, A61K 4300
Patent
active
042384729
ABSTRACT:
The invention provides a double-antibody radioimmunoassay method for the determination of chlorinated dibenzo-p-dioxins, particularly, 2,3,7,8-tetrachlorodibenzo-p-dioxin, in environmental samples including animal tissues such as monkey liver and adipose tissues. The limit of detection is approximately 25 picograms for 2,3,7,8-tetrachlorodibenzo-p-dioxin. Assuming an appropriate cleanup procedure is used, chlorinated dibenzofurans are the only likely interferences, and these can be distinguished through the use of two antisers of different dibenzo-furan/dibenzodioxin selectivities. The invention includes the preparation of a reproducible antigen, an appropriate radiolabeled hapten, and effective sample extracts. A feature of the assay method is the use of a nonionic detergent (e.g., "Cutscum" or "Triton X-305") to solubilize the extremely hydrophobic dibenzo-p-dioxins in a manner permitting their binding by antibodies. The immunoassay is applicable to screening samples in order to minimize the demand for mass spectrometric screening, and to routine monitoring for exposure to known chlorinated dibenzo-p-dioxins in potentially contaminated environments.
REFERENCES:
patent: 3241920 (1966-03-01), Zweig
patent: 3896217 (1975-07-01), Johnson
patent: 3897212 (1975-07-01), Leon et al.
patent: 3985867 (1976-10-01), Redshaw
patent: 4022876 (1977-05-01), Anbar
patent: 4022878 (1977-05-01), Gross
Albro Phillip W.
Chae Kun
Luster Michael I.
McKinney James D.
Nucker Christine M.
Padgett Benjamin R.
United States of America
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