Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1977-10-27
1980-12-02
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 17, 424 1, 424 15, 424 12, C12Q 166, C12Q 150
Patent
active
042372194
ABSTRACT:
Methods and materials for quantitative detection of MB and BB isoenzymes of creatine kinase by radioimmunoassay competitive displacement techniques involving antibodies to human BB isoenzyme which are specific for the B monomer, react with BB CK and cross-react with MB CK, but do not cross-react with MM CK. An acylating agent is employed to radioactively label purified CK isoenzymes used as antigens. Incubation is preferably carried out in a Tris buffer having a pH of about 7.4, in the presence of a suitable organic reducing agent such as mercaptoethanol.
REFERENCES:
patent: 4067775 (1978-01-01), Wurzburg et al.
Charles W. Parker, Radioimmunoassay of Biologically Active Compounds, Prentice-Hall Inc., pp. 93,94 and 126-132; 1976.
Millard N. Caroll et al., New Techniques in Tumor Localization and Radioimmunoassay, John Wiley & Sons, pp. 9-15; 1974.
Fishel Grace J.
Warden Robert J.
Washington University
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