Radioenzymatic method for assaying normetanephrine and octopamin

Details Radioenzymatic method for assaying normetanephrine and octopamin Radioenzymatic method for assaying normetanephrine and octopamin

1984-05-22

1986-05-27

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving transferase

435193, C12Q 148, C12N 910

Patent

active

045915512

ABSTRACT:
An improvement in the method for assaying normetanephrine and octopamine. A procedure is used in which the phenylethanolamine in a sample is enzymatically transmethylated in an incubation mixture of the phenylethanolamine, a compound containing a transferable tritiated methyl group and a transfer enzyme, to form a tritiated N-methyl derivative, and the radioactivity of the derivative is measured. In accordance with the present improvement, incubation of the mixture is conducted at a pH of at least 8.6, preferably in the range of 8.8-9.3. Prior to incubation, as appropriate, the sample may be deproteinized and the phenylethanolamine concentrated.

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Hoertnagl et al., Chem. Abstracts, 87 (1977) #180207a.
Nagel-Hiemke et al., Chem. Abstracts, 95 (1981) #146428d.

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