Radioenzymatic assay of catecholamines

Details Radioenzymatic assay of catecholamines Radioenzymatic assay of catecholamines

1978-10-12

1980-12-30

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

435 15, C12N 910

Patent

active

042424569

ABSTRACT:
A new radioenzymatic assay for catecholamines utilizing the catechol-O-methyl transferase transfer of a methyl group from a labeled methyl donor to the catecholamines followed by isolation of the O-methylated(.sup.3 H)catecholamine and the subsequent measurement of radioactivity.

REFERENCES:
Barman, Enzyme Handbook, vol. 1, Springer-Verlag, New York, Inc., New York (1969), p. 260.
Axelrod, "Catechol-O-Methyl Transferase from Rat Liver", Methods in Enzymology, vol. 5, (1962), Colowick Ed., Academic Press, New York, pp. 748-751.
Raymond et al., "Microassay of Human Erythracyte Catechol-Methyltransferase: Removal of Inhibitory Calcium Ion with Chelating Resin", Chin. Chim. Acta., vol. 58, (1975), pp. 185-194.
Coyle et al., "Catecholamines in Fetal and Newborn Rat Brain", J. of Neurochem., vol. 21, (1973), pp. 61-67.
Passon et al., "A Simplified Radiometric Assay for Plasma Norepinephrine and Epinephrine", Analytical Biochemistry, vol. 51, No. 2, (1973), pp. 618-631.

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