Radioassay of vitamin B-12 employing bentonite

Details Radioassay of vitamin B-12 employing bentonite Radioassay of vitamin B-12 employing bentonite

1974-08-26

1976-02-10

Padgett, Benjamin R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, G01N 3100, G21H 502

Patent

active

039377997

ABSTRACT:
Radioassay for vitamin B-12 using the unknown quantity of non-radioactive vitamin B-12 released from serum mixed with the radioactivity of a known quantity of radioactive vitamin B-12 tracer. A solution of intrinsic factor having a binding capacity less than the quantity of serum vitamin B-12 and radioactive vitamin B-12 is used to bind a portion of the vitamin B-12 mixture. The vitamin B-12 not bound to intrinsic factor is removed by addition of a bentonite-containing tablet. The quantity of radioactive vitamin B-12 bound to intrinsic factor is compared with standard values and the unknown serum vitamin B-12 obtained.
In the steps of the procedure the acid assay medium is pre-combined with the radioactive tracer so that the radioactive vitamin B-12 tracer receives the same treatment as serum vitamin B-12. Certain of the other reagent solutions are pre-combined and the concentration of the components adjusted so that the volume used of each of these other reagent solutions is the same in different assay steps. Thus, fewer pipetting steps are necessary.

REFERENCES:
patent: 3442819 (1969-05-01), Herbert
Hall et al., Radioisotopes in Medicine: in Vitro Studies, U.S. Atomic Energy Commision, June, 1968, pp. 365-379.

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