Radioassay kit for method of determining methotrexate

Details Radioassay kit for method of determining methotrexate Radioassay kit for method of determining methotrexate

1976-12-23

1978-07-25

Padgett, Benjamin R.

Special receptacle or package

Combined or convertible

Packaged assemblage or kit

23253R, 424 1, A61B 1900, A61K 4300

Patent

active

041024559

ABSTRACT:
A radioassay system for the determination of methotrexate in biological fluids based on the competitive binding of labeled and unlabeled methotrexate to the enzyme dihydrofolate reductase. Samples of unknown methotrexate level are mixed with I.sup.125 labeled methotrexate. A portion of the total methotrexate present is bound by the addition of enzyme, and the unbound methotrexate is removed with charcoal. The level of bound I.sup.125 labeled methotrexate is measured in a gamma counter. To calculate the methotrexate level of the unknown samples, the displacement of bound labeled methotrexate caused by the unknowns is compared to the displacement caused by known methotrexate standards.

REFERENCES:
patent: 3981983 (1976-09-01), Caston et al.
patent: 3989812 (1976-11-01), Barrett et al.
patent: 4043759 (1977-08-01), Charm et al.
Croll et al., Ed., New Techniques in Tumor Location and Radio Immuno Assay, John Wiley & Sons, N.Y., 1974.

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