Radioassay for vitamin B.sub.12

Details Radioassay for vitamin B.sub.12 Radioassay for vitamin B.sub.12

1979-03-23

1982-06-08

Nucker, Christine M.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

23230B, 424 12, G01N 3354, G01N 3356, G01T 100

Patent

active

043339182

ABSTRACT:
The amount of vitamin B.sub.12 in solution in a liquid is determined by mixing a sample of the solution with vitamin B.sub.12 -binding proteins from chicken serum in a pH of about 12.8 to 13.2, and then analyzing a component of the mixture. In preferred procedures, especially useful in assaying human sera, a competitive binding technique is employed utilizing labelled vitamin B.sub.12 and a buffer containing cyanide ion. By operating at very high pH and with chicken serum proteins, the assay can be conducted relatively simply and with very accurate results.

REFERENCES:
patent: 3937799 (1976-02-01), Lewin et al.
patent: 4146602 (1979-03-01), Gutcho et al.
patent: 4188189 (1980-02-01), Allen

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