Chemistry: analytical and immunological testing – Biospecific ligand binding assay – Utilizing isolate of tissue or organ as binding agent
Patent
1981-08-26
1984-09-04
Gron, Teddy S.
Chemistry: analytical and immunological testing
Biospecific ligand binding assay
Utilizing isolate of tissue or organ as binding agent
424 11, 436 63, 436 71, G01N 3350, G01N 3360
Patent
active
044697953
ABSTRACT:
A radioassay test for the rapid and sensitive determination of the concentration of monosialoglycosphingolipid G.sub.M1 ganglioside concentrations in small volumes of cerebrospinal fluid from individual patients is based on the high affinity interaction between cholera enterotoxin and G.sub.M1 ganglioside. The level of G.sub.M1 ganglioside present in the cerebrospinal fluid has been shown to be an indicator of active central nervous system pathology. In the present invention, the use of highly specific toxins eliminates cross reactivity with the other gangliosides, thereby eliminating the need to process the cerebrospinal sample prior to assay, except for centrifugation. Results of a specific application of the subject radioassay to newborn infants and older infants and children (some of the latter having active neurologic disease) indicated that the G.sub.M1 ganglioside concentration in the cerebrospinal fluid is probably a function of both central nervous system tissue ganglioside content and turnover.
REFERENCES:
patent: 4125492 (1978-11-01), Cuatrecasas et al.
patent: 4225487 (1980-09-01), Cuatrecasas et al.
Pediatric Research, vol. 14(11) pp. 1276-1279(1980) Ginns et al.
Proc. Society Experimental Biology & Medicine, vol. 145(4), pp. 1187-1191, (1974) Peterson et al.
J. Neurochemistry, vol. 35(4), pp. 977-982 (1980), Ginns, E. and French, J.
French Joseph
Ginns Edward
Gron Teddy S.
Moskowitz M.
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