Radioactivity detection in high-pressure liquid chromatography

Radiant energy – Automatic/serial detection of similar sources

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250367, G01T 100, G01T 120

Patent

active

042674514

ABSTRACT:
In a high-pressure liquid chromatography system, a radioactive sample in the effluent from a chromatographic column can be detected by passing the effluent into a cell packed with a fluorescent composition comprising a scintillation material chemically bonded to an inert substrate. A suitable fluorescent composition is microparticulate didansyl-N-2-aminoethyl-3-aminopropyl silica. Radioactive emissions from the sample cause scintillations in the fluorescent composition that can be detected through the walls of the cell. In order to obtain a chromatogram of the radioactivity occurring in the column, aliquots of the effluent from the column may be passed sequentially into a plurality of cells arranged on a carousel support structure that is rotable in stepwise fashion so that each cell passes in turn through a fill station, a detection station, and a wash station. At the fill station, each cell in turn is filled with an aliquot of effluent. As one cell is being filled at the fill station, the scintillations occurring in another cell are being monitored at the detection station, while still another cell is being washed at the wash station in order to remove the aliquot of effluent previously monitored for radioactivity. After being washed, each cell can subsequently be filled with a fresh aliquot of effluent at the fill station.

REFERENCES:
patent: 3898460 (1975-08-01), Noakes et al.
patent: 4019372 (1977-04-01), Parkell et al.

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