Radioactive staining of gels to identify proteins

Details Radioactive staining of gels to identify proteins Radioactive staining of gels to identify proteins

1982-02-11

1984-07-10

Hunt, Brooks H.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

C09K 1104

Patent

active

044593562

ABSTRACT:
Radioactive staining of gels is employed to identify proteins. A radioactive stain composition is formed by introducing radioactive iron isotope into ferrous bathophenanthroline sulfonate by adding crystalline ascorbic acid and acetic acid to a .sup.59 FeCl.sub.3 solution, adding aqueous BPS solution after the dissolution of the crystals, and adding methanol and acetic acid to yield the radioactive stain composition. Alternatively, the radioactive stain composition may be formed by bombarding a non-radioactive stain with thermal neutrons. The method of identifying proteins with the radioactive stain composition basically comprises the steps of placing the protein in the gel, forming a radioactic stain composition, and applying the radioactivated stain composition to the gel. To demonstrate the protein present, either a gamma scintillation counting technique or radioautography is preferably employed.

REFERENCES:
patent: 3673410 (1972-06-01), Waite et al.
patent: 3678148 (1972-07-01), Caiola
patent: 3773467 (1973-11-01), Yang et al.
patent: 3801783 (1974-04-01), Caiola
patent: 3856930 (1974-12-01), Nodine et al.
patent: 3887332 (1975-06-01), Takase et al.
patent: 3925020 (1975-12-01), Ogawa et al.
patent: 3959455 (1976-05-01), Ansari et al.
patent: 4129644 (1978-12-01), Kalopissis et al.
patent: 4219337 (1980-08-01), Grossberg et al.
patent: 4256727 (1981-03-01), Triplett et al.
Kobodera, A. et al., Radioisotopes, vol. 23, pp. 628-634 (1974).
Zapolski, E. J. et al., Analytical Biochemistry, vol. 123, pp. 325-328.
Merril et al., "Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows . . . ", Science, vol. 211 (3/27/81), pp. 1437-1438.
O'Farrell, "High Resolution Two-Dimensional Electrophoresis of Proteins," J. Biol. Chem., vol. 250 (5/27/75), pp. 4007-4021.
Elder et al., "Radioiodination of Proteins in Single Polyacrylamide Gel Slices," J. Biol. Chem., vol. 252 (9/25/77), pp. 6510-6515.
Graham et al., "Polyacrylamide Gel Staining with Fe.sup.2+ -Bathophenanthroline Sulfonate," Anal. Biochem., vol. 88 (1978), pp. 434-441.
Poehling et al., "Visualization of Proteins with a Silver `Stain`: A Critical Analys," Electrophoresis, vol. 2 (1981), pp. 141-147.
Laemmli, U. K., "Cleavage of Structural Proteins During the Assembly . . . ", Nature, vol. 227 (8/15/70), pp. 680-681.
Sammons et al., "Ultrasensitive Silver-Based Color Staining of Polypeptides in . . . ", Electrophoresis, vol. 2 (1981), pp. 135-141.

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