Radiation dosimeter and method of making and using

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435 6, 435 911, 4352872, 436 94, 536 231, 536 243, 536 2433, 536 253, C12Q 168, C12P 1934, C12M 100, C07H 2104

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active

058916822

ABSTRACT:
A device for measuring a total dose of biologically effective radiation is provided, in a specific embodiment, for measuring ultraviolet B radiation (UVB) in the wavelength range of 290-400 nm. X-radiation and high-energy particle radiation are also measurable with this device. The device includes a sample of DNA dried onto a plastic film, and the analysis method includes assaying for damage with the use of polymerase chain reaction and fluorescence techniques. The damage to the DNA is correlatable with dosage via a comparison with standardized calibration data.

REFERENCES:
"DNA as a Solar Dosimeter in the Ocean,"J.D. Regan, et al., Photochemistry and Photobiology, vol. 56, No. 1, pp. 35-42.
"Journal of Photochemistry and Photobiology,"B. Biology, James D. Regan, et al., Department of Biological Sciences and Claude Pepper Institute, Florida Institute of Technology, Melbourne, Florida, Published Dec. 11, 1995, pp. 57-61.

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