RAD51 polypeptides

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease

Reexamination Certificate

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C435S424000, C800S298000, C800S320100

Reexamination Certificate

active

06905857

ABSTRACT:
Nucleic acid sequences encoding two RAD51 recombinases active in maize plants are provided. cDNA sequences including the ZmRAD51 coding sequences and unique 3′-untranslated regions which are useful as RFLP probes, are also provided. The production of plasmids containing a nucleic acid sequence encoding a ZmRAD51 fusion protein, as well as the use of the plasmids to introduce the ZmRAD51 coding sequence into a host cell, such as maize cell, are also disclosed.

REFERENCES:
patent: WO 93/22443 (1993-11-01), None
patent: WO 97/41228 (1997-11-01), None
Kowalczykowski, et al. 1994.Homologous Pairing and DNA Strand-Exchange Proteins. Annu. Rev. Biochem. 1994. 63.991-1043.
Sung, 1994.Catalysis of ATP-Dependent Homologous DNA pairing and Strand Exchange by Yeast RAD51 Protein. Science, vol. 265, 1241-1243.
Stassen, et al.Isolation and characterization of rad51 orthologs from Coprinus cinereous and Lycopersicon esculentum, and phylogenetic analysis of eukaryotic recA homologs. Curr Genet (1997) 31: 144-157.
Doutriaux, et al.Isolation and characterisation of the RAD51 and DMC1 homologs from Arabidopsis thaliana. Mol Gen. Genet (1998) 257: 283-291.
Kanaar and Hoeijmakers, 1998.From competition to collaboration. Nature, vol. 391, 335-338.
Benson, et al. 1998.Synergistic actions of Rad51 and Rad52 in recombination and DNA repair. Nature, vol. 391, 401-404.
Shinohara and Ogawa, 1998.Stimulation by Rad52 of yeast Rad51-mediated recombination. Nature, 391: 404-407.
New, et al., 1998.Rad 52 protein stimulates DNA strand exchange by Rad51 and replication protein A. Nature, 391: 407-410.
Smith, K.N., et al. 1996.Untitled. Embl. Sequence Data Library, XP002105502, Accession No. U43528.

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