Organic compounds -- part of the class 532-570 series – Organic compounds – Heterocyclic carbon compounds containing a hetero ring...
Patent
1997-05-30
1998-07-28
Rotman, Alan L.
Organic compounds -- part of the class 532-570 series
Organic compounds
Heterocyclic carbon compounds containing a hetero ring...
514330, C07D21130, A61K 31445
Patent
active
057864845
DESCRIPTION:
BRIEF SUMMARY
This application is a 371 of PCT/GB95/02247 which is now published as WO96/09290.
1. Field of the Invention
This invention relates to the racemisation and dynamic resolution of optically-enriched heterocyclic carboxanilides, e.g. piperidine-2-carboxanilides such as levobupivacaine.
2. Background of the Invention
Compounds of formula 1 (see formulae, below) wherein R.sup.1 is methyl, n-propyl, n-butyl or cyclopropyl and R.sup.2 is a 2,6-dimethylphenyl have utility as local anaesthetics. Biological studies have shown that (S)-enantiomers of such compounds display lower cardiotoxicity than the corresponding racemates whilst maintaining the same anaesthetic potency, and are therefore potentially more beneficial for clinical uses. Thus there is a requirement for efficient processes to manufacture compounds of formula 1 in the form of single enantiomers. For this purpose, conventional resolution approaches invariably afford up to 50% of the unwanted enantiomer. To improve atom utilisation in such processes, it is desirable to recycle the unwanted enantiomer by effecting its racemisation to provide material suitable for subsequent resolution. Additional benefits may be attainable by "asymmetric transformation", comprising simultaneous racemisation and crystallisation-induced resolution in a one-pot process.
Fyhr et al, Acta Pharm. Suecica 25(3):121-132 (1988), disclose the racemisation of ropicavaine hydrochloride (1.HCl, R.sup.1 =n-propyl, R.sup.2 =2,6-dimethylphenyl, absolute configuration =S) in dilute aqueous solution at pH 1-6, using HCl, and 80.degree.-130.degree. C. The results are presented as a preformulation stability study and merely serve to indicate that ropivacaine racemises slowly in aqueous media.
Shiraiwa et al, Bull. Chem. Soc. Jpn. 64:3251-3255 (1991), disclose asymmetric transformation of 2-piperidine-carboxylic acid, by heating in an alkanoic acid solvent in the presence of an chiral acid resolving agent and an aldehyde. The latter component is believed to assist racemisation by formation of a cationic Schiff base intermediate, a mechanistic pathway which can also operate on piperidine-2-carboxanilides 1 only in cases where R.sup.1 =H. Again, this process is unsuitable for operation on a manufacturing scale, not least because it uses environmentally-unacceptable reagents.
SUMMARY OF THE INVENTION
The present invention is based on the surprising discovery that N-heterocyclic-2-carboxanilides, including compounds of formula 1 wherein R.sup.1 is H, methyl, n-propyl, n-butyl or cyclopropyl and R.sup.2 is phenyl optionally substituted with one or more methyl groups, undergo rapid racemisation when heated in solution in the presence of a carboxylic acid R.sup.3 CO.sub.2 H wherein R.sup.3 is either n-alkyl or aryl (exemplified in Scheme 1) or any acid having a pKa of -1 to +6, relative to water. The reaction can be carried out in a wholly or substantially non-aqueous system, e.g. either in a solution of neat acid or in the presence of an inert cosolvent such as xylene or toluene. The presence of residual salt forms of compounds of formula 1, e.g. as the result of resolution using a chiral resolving agent, do not impede the efficiency of the process.
A preferred embodiment of the invention is the racemisation of bupivacaine (1, R.sup.1 =n-butyl, R.sup.2 =2,6-dimethylphenyl) enriched in one enantiomer, preferably the (R)-enantiomer, by heating with propanoic acid or butanoic acid. A suitable cosolvent such as xylene allows the reaction to be conducted at optimum temperature, i.e. about 130.degree. C. Compared to the prior art, this process, and processes of the invention in general, afford significant advantages since neither dilute aqueous solutions
As a further feature of the invention, it has been discovered that asymmetric transformation of the N-heterocyclic-2-carboxanilides can be achieved by including a chiral acid resolving agent as an additional component in the processes described above (exemplified in Scheme 2).
Two variants of such transformations are possible: firstly, a one-pot proces
REFERENCES:
Fyhr, P. and Hogstrom, C.: A Preformulation Study on the Kinetics of the racemization of Ropivacaine hydrochloride Acta Pharm. Suecica vol. 25(3), pp. 121-132, 1988.
Shiraiwa, T. et al.: Asymmetric Transformations of Proline and 2-Piperidinecarboxylic Acid via Formation of Salts with Optically Active Tartaric Acid Bull. Chem. Soc. Jpn., vol. 64, pp. 3251-3255, 1991.
Dyer Ulrich Conrad
Lock Christopher James
Woods Martin
Aulakh Charanjit S.
Chiroscience Limited
Rotman Alan L.
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