R2D2: an enzyme of RNA silencing

Details R2D2: an enzyme of RNA silencing R2D2: an enzyme of RNA silencing

2006-12-05

2006-12-05

McGarry, Sean (Department: 1635)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S006120, C435S069100, C536S024500

Reexamination Certificate

active

07144714

ABSTRACT:
Methods for making siRNA comprise recombinantly coexpressing a Dicer protein with an R2D2 protein to form a complex comprising the R2D2 protein and the Dicer protein, and contacting the complex with a double-stranded (ds) RNA under conditions wherein the complex cleaves the dsRNA into siRNA. The R2D2 protein may be aDrosophilaR2D2 protein and the Dicer protein may be aDrosophilaDicer-2 protein. The Dicer protein and the R2D2 protein may be coexpressed in insect cells, such as S2, Sf9 or Hi5 cells, using a baculovirus expression system.

REFERENCES:
Liu et al., 2003 Science 301:1921-1925.
Tomari et al., 2004 Science 306:1377-1380.
Meister et al., 2004, Nature 431:343-349.
Pellino et al., 2003, Cell 115(2):132-133).
Bernstein et al., 2001, Nature 409:363-6.
Thornton et al., 2000, Nature Struct. Biol. 7 (Suppl.)991-994.
Gerwitz et al. (1998) Blood 92(3):712-736.
Greco et al. (2002) Frontiers in Bioscience 7:d1516-1524.

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