Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing nitrogen-containing organic compound
Reexamination Certificate
2004-02-24
2009-08-11
Nashed, Nashaat T (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing nitrogen-containing organic compound
C435S232000
Reexamination Certificate
active
07572608
ABSTRACT:
The invention relates to R-hydroxynitrillyases from the family of Rosaceae that are characterized by an improved substrate tolerance and increased stability. In the active center of the R-hydroxynitrillyases either a) an alanine group is substituted by glycine, valine, leucine, isoleucine, or phenylalanine or b) a phenylalanine group is substituted by alanine, glycine valine, leucine or isoleucine, or c) a leucine group is substituted by alanine, glycine, valine, isoleucine or phenylalanine, or d) an isoleucine group is substituted by alanine, glycine, valine, leucine or phenylalanine. The invention also relates to the use of these lyases in the production of enantiomer-pure R- or S-cyanohydrines.
REFERENCES:
patent: 6319697 (2001-11-01), Effenbrger et al.
patent: 6861243 (2005-03-01), Schwab et al.
patent: 0 969 095 (2000-01-01), None
patent: 1 223 220 (2002-07-01), None
Glieder et al.(Angew. Chem.. Intl. ed. 2003, 42, 4815-4818).
Glieder et al. (Ang chem. Intl ed. 42, 4815-4818, 2003), In IDS.
Whisstock, et al. Quarterly Rev. Biophy. 2003, 36, pp. 307-340.
Ingrid Dreveny et al, The active site of hydroxynitrile lyase fromPrunus amygdalus: Modeling studies provide new insights into the mechanism of cyanogenesis; Feb. 2002, pp. 292-300, XP-002281486.
Ingrid Dreveny et al, The hydroxynitrile lyase from Almond: A Lyase that looks like an oxidoreductase; Sep. 2001; pp. 803-815; XP-002281487.
Herfried Griengl et al, The synthesis of chiral cyanohydrins by oxynitrilases; Jun. 2000; pp. 252-256; XP-002249789.
Anton Glieder et al, Comprehensive Step-by-Step Engineering of an (R)-Hydroxynitrile Lyase for Large-Scale Asymmetric Synthesis; Oct. 13, 2003; pp. 4815-4818; XP-002281488.
Glieder Anton
Gruber Karl
Luiten Ruud
Skranc Wolfgang
Weis Roland
DSM Fine Chemicals Austria NFG GmbH & Co. KG
Meah Md. Younus
Nashed Nashaat T
Nixon & Vanderhye P.C.
LandOfFree
R -hydroxynitrillyases having improved substrate tolerance... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with R -hydroxynitrillyases having improved substrate tolerance..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and R -hydroxynitrillyases having improved substrate tolerance... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4118886