Organic compounds -- part of the class 532-570 series – Organic compounds – Nitrogen attached directly or indirectly to the purine ring...
Reexamination Certificate
2005-07-15
2010-10-19
Wilson, James O (Department: 1624)
Organic compounds -- part of the class 532-570 series
Organic compounds
Nitrogen attached directly or indirectly to the purine ring...
Reexamination Certificate
active
07816527
ABSTRACT:
Kits including novel fluorescent derivitization reagents for labeling an aldehyde- and/or ketone-containing target substance in a sample are described. In one embodiment kits are provided the novel fluorescent derivitization reagents of which have the following formula:wherein Q is carbonyl, thiocarbonyl, or sulfonyl, and R5is -L-Z; L is arylene, or a C1-6perfluoroalkylene; Z is a carbonyl hydrazide, hydrazide, sulfonyl hydrazide, or a thiocarbonyl hydrazide; R11-R14are independently H, C1-16alkyl, C1-6alkylamino, amino, or halogen; and R21-R24are independently H, C1-6alkyl, C1-6alkoxy, C1-6perfluoroalkyl, C1-6alkylamino, di(C2-12-alkyl)amino, amino, carboxy, cyano, halogen, hydroxy, nitro, phenyl, sulfo, or -L-Z. The kits and reagents described are particularly useful for labeling glycoproteins or glycopeptides, nucleic acids, and lipopolysaccharides in electrophoresis gels.
REFERENCES:
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Garcia-Campaña, et al. J. Chromatogr. B, 793, 2003, pp. 49-74.
Sauer, et al. J. Fluoresc. 3, 1993, pp. 131-139.
Dörwald, F. Zaragoza. Side Reactions in Organic Synthesis: A Guide to Successful Synthesis Design, Weinheim: Wiley-VCH Verlag GmbH & Co. KGaA, 2005, Preface.
Dimitrova, et al. Dokladi na Bulgarskata Akademiya na Naukite, 51(9-10), 1998, 99-102 (Abstract only).
Diwu Zhenjun
Haugland Richard P.
Patton Wayne F.
Steinberg Thomas H.
Life Technologies Corporation
Willis Douglas M
Wilson James O
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