Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Via microinjection of a nucleus into an embryo – egg cell – or...
Patent
1997-02-19
2000-11-14
Crouch, Deborah
Multicellular living organisms and unmodified parts thereof and
Method of making a transgenic nonhuman animal
Via microinjection of a nucleus into an embryo, egg cell, or...
800 8, 800 14, 435325, A01K 6700, C12N 1500
Patent
active
06147276&
ABSTRACT:
A method of reconstituting a mammalian embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more mammals. The invention is useful in the production of transgenic mammals as well as non-transgenics of high genetic merit.
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Campbell Keith Henry Stockman
Wilmut Ian
Biotechnology & Biological Sciences Research Council
Crouch Deborah
Earp David J.
Roslin Institute (Edinburgh)
The Minister of Agriculture, Fisheries & Food
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