Quick assay for detection of salmonella

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 29, 435 30, 435 31, 435 34, 435 39, 435295, C12Q 110, C12Q 102, C12Q 124, C12Q 122

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active

052120678

ABSTRACT:
A swift, accurate assay for the detection of salmonella contamination in a livestock environments, comprises sampling the floor of the livestock holding area with drag swabs for about 10 or more minutes, after which said swabs are maintained in a static condition, at reduced temperature, in the presence of double strength skim milk, until ready for testing. The swabs are transferred to a salmonella-preferential growth medium, such as tetrathionate broth and subsequently assayed. To reduce non-salmonella "look alike" or masking bacterial colonies, Novobiocin may be administered to the culture media.

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Difco Manual, 10th Edition 1984, pp. 947-949.
Julseth et al. (1964) Effect of temperature on--Appl. Microbiol 17:767-768.
Dega et al. (1972) Growth of Salmonella--Appl. Microbiol 23:82-87.

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