Quenching reagents and assays for enzyme-mediated luminescence

Details Quenching reagents and assays for enzyme-mediated luminescence Quenching reagents and assays for enzyme-mediated luminescence

1995-06-07

1998-04-28

Leary, Louise

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving luciferase

435 4, 435975, 435963, 435 23, 25230116, 252646, 356 401, C12Q 166, C12Q 100, C12Q 137, C09K 1106

Patent

active

057443203

ABSTRACT:
The present invention relates to single and dual-reporter luminescence assays utilizing general and specific reagents to quench enzyme-mediated reactions. In one embodiment of the invention, a reagent is added to the assay which non-specifically quenches enzyme-mediated luminescent reactions. In another embodiment of the invention, a reagent is added to the assay which simultaneously quenches one enzyme-mediated luminescent reaction while activating another distinct enzyme-mediated luminescent reaction. An assay kit containing specific quench reagents, and the reagents themselves are also disclosed.

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