Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-09-07
1998-03-17
Houtteman, Scott W.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
564281, C07H 2100
Patent
active
057288224
ABSTRACT:
The present invention discloses a novel method for isolating RNA from biological samples, most particularly blood, using selected quaternary amine surfactants. The RNA is isolated quickly and in sufficient quantity and quality to permit analysis by methods including reverse transcriptase and polymerase chain reaction.
REFERENCES:
patent: 3620807 (1971-11-01), Murray
patent: 3962332 (1976-06-01), Trapasso
patent: 4083872 (1978-04-01), Schwarze et al.
patent: 4843155 (1989-06-01), Chomczynski
patent: 4935342 (1990-06-01), Seligson et al.
patent: 5010183 (1991-04-01), Macfarlane
patent: 5171641 (1992-12-01), Roberts et al.
1988 Stratagene Catalog, p. 39.
Waernheim, et al. A comparison between the organic solution phases containing hexanol, water . . . Acta. Chem. Scand., Ser. A (1986) vol. A40, Dialog No. 105067011.
Biresaw, et al. Dynamic light scattering study of a micellar system of low fractional ionization . . . J. Phys. Chem. (1985) 89 :5144-5146.
Yatsimirsky et al. Kinetics of the axial ligation of aquacobaloximes in the presence of . . J. Chem. Soc., Faraday Trans. 1 (1986) 82 :319-327.
Japanese Patent No. 61078796, published Apr. 22, 1986, Sagami Chemical Research Centre, Abstract.
Chirgwin et al, "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease", Biochem., 18(24) :5294-5299 (1979).
D. M. Wallace, "Large-and Small-Scale Phenol Extractions", Meth. Enzym., 152:33-41 (1987).
P. Chomczynski et al, "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction", Anal. Biochem., 162:156-159 (1987).
T. Maniatis et al, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory (1982).
L. G. Davis et al, "Guanidine Isothiocyanate Preparation of Total RNA" and RNA Preparation: Mini Method in Basic Methods in Molecular Biology, Elsevier, New York, pp. 130-138 (1991).
R. Kingston et al, "Preparation and Analysis of RNA" in Current Protocols in Molecular Biology, Unit 4.2 (Supplement 14), ed. F. M. Ausubel et al, John Wiley, (1991).
G. Seibert et al, "The Separation of High and Low Molecular Weight RNA by Precipitation with N-Cetyl-N,N,N-trimethylammoniumbromide", Z. Naturforsch Sect. C Biosci., 32(3-4) :294-296 (1977).
Tokyo Organic Chemical Industries, Ltd., "Carbamate Pesticide Composition", Chemical Abstracts, 95(23) :199031, Abstract No. 95:199031a (1981).
A. Nakayama et al, "Antiseptic Detergent", Chemical Abstracts, 52(10:3376, Abstract Col. No. 3376h (1958).
A. Nakayama et al, "Quaternary Ammonium Salts of Dithiocarbamic Acid", Chemical Abstracts 52(10) :8194, Abstract Col. No. 8194c (1958).
Kirk-Othmer, "Sulfonation and Sulfation to Thorium and Thorium Compounds" ed. J. Wiley & Sons, Encyclopedia of Chemical Technology, 22(3) :383-385 (1983).
Houtteman Scott W.
Qiagen N.V.
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