Quaternary amine surfactants and methods of using same in isolat

Details Quaternary amine surfactants and methods of using same in isolat Quaternary amine surfactants and methods of using same in isolat

1993-02-01

1994-04-05

Parr, Margaret

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

564281, C07H 2100

Patent

active

053006356

ABSTRACT:
The present invention discloses a novel method for isolating nucleic acids from biological samples, most particularly blood, using selected quaternary amine surfactants. The nucleic acids are isolated quickly and in sufficient quantity and quality to permit analysis by methods including reverse transcriptase and polymerase chain reaction.

REFERENCES:
patent: 4843155 (1989-06-01), Chomczynski
patent: 5010183 (1991-04-01), Macfarlane
Seibert, G. et al. The Separation of High and Low Molecular Weight RNA by Precipitation . . . , Z., Naturforsch Sect C Biosciences (1977) 32: 294-296.
Chomczynski, P. et al. Single-Step Method of RNA Isolation by Acid Quanidium . . . Anal. Biochem (1987) 162:156-159.
Chirgwin et al, "Isolation of Biologically Active Ribonucleic Acid from Sources Enriched in Ribonuclease", Biochem., 18(24):5294-5299 (1979).
D. M. Wallace, "Large- and Small-Scale Phenol Extractions", Meth. Enzym., 152:33-41 (1987).
T. Maniatis et al, Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory (1982).
L. G. Davis et al, "Guanidine Isothiocyanate Preparation of Total RNA" and RNA Preparation: Mini Method in Basic Methods in Molecular Biology, Elsevier, New York, pp. 130-138 (1991).
R. Kingston et al, "Preparation and Analysis of RNA" in Current Protocols in Molecular Biology, Unit 4.2 (Supplement 14), ed. F. M. Ausubel et al, John Wiley, (1991).

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