Active solid-state devices (e.g. – transistors – solid-state diode – Regenerative type switching device
Reexamination Certificate
2008-07-29
2008-07-29
Lindsay, Jr., Walter (Department: 2812)
Active solid-state devices (e.g., transistors, solid-state diode
Regenerative type switching device
C257S205000, C257S227000, C438S014000, C977S700000, C977S774000
Reexamination Certificate
active
07405434
ABSTRACT:
A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.
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Craighead Harold G.
Edel Joshua B.
Samiee Kevan T.
Stavis Samuel M.
Cornell Research Foundation Inc.
Lindsay, Jr. Walter
Mustapha Abdulfattah
Schwegman Lundberg & Woessner, P.A.
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