Drug – bio-affecting and body treating compositions – Radionuclide or intended radionuclide containing; adjuvant... – Molecular bilayer structure
Patent
1979-08-24
1982-09-28
Nucker, Christine M.
Drug, bio-affecting and body treating compositions
Radionuclide or intended radionuclide containing; adjuvant...
Molecular bilayer structure
23230B, 424 12, G01N 3356, G01N 3358
Patent
active
043518222
ABSTRACT:
The vitamin B.sub.12 (cobalamin) level of human blood and mammalian tissue is routinely assayed utilizing radioisotope dilution assay (RIDA) techniques. The presence of vitamin "B.sub.12 analogues" in most such samples, which analogues are erroneously assayed as true vitamin B.sub.12 utilizing prior art RIDA techniques, has been determined. Such errors are due to the fact that binding proteins commonly used in prior art RIDA techniques usually include proteins which bind both true vitamin B.sub.12 and the previously unrecognized vitamin B.sub.12 analogues which are present in such samples. The errors previously caused by the measurement of vitamin B.sub.12 analogues are avoided by using an assay composition for binding vitamin B.sub.12 which is substantially free of substances which bind vitamin B.sub.12 analogues in said samples. Methods of making and using such assay compositions are taught.
REFERENCES:
patent: 3434927 (1969-03-01), Highley
patent: 3442819 (1969-05-01), Herbert
patent: 3591678 (1971-07-01), Ellenbogen et al.
patent: 3937799 (1976-02-01), Lenin
patent: 4188189 (1980-02-01), Allen
Allen, 16th Int. Cong. Hemotol., Abstracts, p. 152, (5-50-4), Sep. 1976.
Kon, Biochem. Soc. Symp. Cambridge, Eng., pp. 17-35, (vol. 13, 1953).
Allen et al., J. Biol. Chem., 3660-3669, 3670-3680, (vol. 248, 1973).
Kolhouse et al., N. Eng. J. Med., 785-792, (vol. 299, 1978).
Kolhouse et al., J. Clin. Inv., 1381-1392, (vol. 60, 1977).
Cohen et al., J. Am. Med. Ass., 1942-1945, (vol. 244, 1980).
Hippe et al., Scan. J. Clin. Lab. Inv., 577-582, (vol. 35, 1975).
Mollin et al., Clinics Haemotol., 521-546, (vol. 5, 1976).
Kondo et al., Proc. Natl. Acad. Sci. USA, pp. 817-821, (vol. 77, 1980).
Glass, Gastric Intrinsic Factor and Other Vitamin B.sub.12 Binders, Part B, Non-IF Binders, George Thieme Publishers, Stuttgart, 1974.
Margolis Donald W.
Nucker Christine M.
University Patents Inc.
LandOfFree
Quantitative testing for vitamin B.sub.12 does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Quantitative testing for vitamin B.sub.12, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Quantitative testing for vitamin B.sub.12 will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-399577