Quantitative retinol assay for serum and dried blood spots

Details Quantitative retinol assay for serum and dried blood spots Quantitative retinol assay for serum and dried blood spots

1995-07-27

1996-07-02

Snay, Jeffrey R.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436131, 436172, 436175, G01N 2164, G01N 3350

Patent

active

055321666

ABSTRACT:
Capillary Zone Electrophoresis (CZE) with laser-excited fluorescence detection has been found to be a fast, easy, and accurate method for directly measuring serum retinol. Due to its small sample requirements, the method may be used for finger-prick analysis.

REFERENCES:
J. Glover, Fluorescence Assay of Retinol-Binding Holoprotein, Methods in Enzymology, 67:282-287 (1980).
HC Furr, A Direct Microassay for Serum Retinol (Vitamin A Alcohol) by Using Size-Exclusion High-Pressure Liquid Chromatography with Fluorescence Detection, Analytical Biochemistry, 171:360-365 (1988).
P. Grossman, Application of Free-Solution Capillary Electrophoresis to The Analytical Scale Separation of Proteins and Peptides, Analytical Chemistrym, 61:1186-1194 (1989).
Kuhr, Indirect Fluorescence Detection of Native Amino Acids in Capillary Zone Electrophoresis, Analytical Chemistry, 60:1832-1834 (1988).
Ma, Fast Minimicrocroassay of Serum Retinol (Vitamin A) by Capillary Zone Electrophoresis with Laser-Excited Fluorescence Detection, Journal of Chromatography, 616:31-37 (1993).

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