Quantitative protein analysis by immunodiffusion

Details Quantitative protein analysis by immunodiffusion Quantitative protein analysis by immunodiffusion

1979-11-27

1981-01-13

Prescott, Arthur C.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204180S, 204180G, 23230B, 424 12, 356 39, 356 72, 356344, G01N 2726, G01N 3316, G01N 2100

Patent

active

042448034

ABSTRACT:
Methods and apparatus are described by which immunochemical procedures such as immunodiffusion and immunoelectrophoresis can be used to provide quantitative measurement of the concentration of individual proteins in fluids such as serum, spinal fluid, tissue extracts and the like. That is accomplished typically by photooptically scanning the zones of precipitation which are produced, in parallel with those of standard preparations containing known concentrations of the proteins to be determined; deriving from the arrays of measurements of the precipitation zones selected parameters; and comparing the parameter values of the experimental and reference preparations. The zone measurements are preferably made and recorded electronically, with suitable digital manipulation of the resulting video values at a plurality of selected positions for each zone.

REFERENCES:
patent: 3096137 (1963-07-01), Silard
patent: 3553067 (1971-01-01), Dwyer et al.
patent: 4012634 (1977-03-01), Bouton et al.
patent: 4070113 (1978-01-01), Frazer et al.
patent: 4159895 (1979-07-01), Shine

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