Quantitative PCR using blocking oligonucleotides

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 243, 536 2433, C12Q 168, C12P 1934, C07H 2104

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active

060338547

ABSTRACT:
Disclosed is a method for determining the number of copies of a target nucleic acid relative to the number of copies of a reference nucleic acid in a biological specimen. The method includes amplifying the target nucleic acid, measuring the amplification of the target nucleic acid, amplifying the reference nucleic acid, measuring the amplification of the target nucleic acid, and comparing the amplification of the target nucleic acid sequence to the amplification of the reference nucleic acid sequence.

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