Quantitative immunochromatographic assays

Details Quantitative immunochromatographic assays Quantitative immunochromatographic assays

1996-03-29

1998-05-19

Achutamurthy, Ponnathapura

Chemistry: analytical and immunological testing

Involving diffusion or migration of antigen or antibody

436534, 435 71, 422 61, G01N 35558, G01N 33546, G01N 3353

Patent

active

057535175

ABSTRACT:
Quantitative immunochromatographic assays for measuring the amount of an analyte, and an apparatus for use in the assays, are disclosed. The assays involve obtaining a fluid sample which contains the analyte; supplying a RAMP.TM. apparatus which includes a membrane having an application point, a detection zone, and a contact region, where the contact region is between the application point and the detection zone, and has antibody-coated particles imbedded within it; contacting the application point with the fluid sample; maintaining the RAMP.TM. apparatus under conditions sufficient to allow fluid to transport analyte by capillary action to the contact region, where the analyte binds to the antibody-coated particles; further maintaining the apparatus under conditions sufficient to allow fluid to transport analyte-bound antibody-coated particles to the detection zone, where they interact with a detection reagent; and detecting the amount of analyte-bound antibody-coated particles which have reacted with the detection reagent. Alternatively, the fluid sample can be contacted with the detection zone of the apparatus, and the antibody-coated particles are mobilized by addition of fluid to the application point. The RAMP.TM. apparatus can additionally include one or more of the following features: a wicking pad; an application pad covering the application point; a contact pad covering the contact region; a separator pad between the membrane and the contact pad; and an internal control.

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