Quantitative determination of the surface charge of serum lipopr

Details Quantitative determination of the surface charge of serum lipopr Quantitative determination of the surface charge of serum lipopr

1980-08-15

1982-05-04

Schor, Kenneth M.

Chemistry: physical processes

Physical processes

Crystallization

23909, 424 7, G01N 3350

Patent

active

043280005

ABSTRACT:
Disclosed is the polyelectrolytic titration of serum lipoproteins, the negative surface charge of the latter being determined quantitatively by adding an excess amount of a polycation in aqueous solution to the particular serum lipoprotein and then back-titrating the positive charge, of the polycation, which has not been neutralized by the negative surface charge, against a chromotropic polyanion, in the presence of a metachromatic dye as the indicator. The method according is used for the diagnosis of lipometabolic disorders.

REFERENCES:
patent: 3884638 (1975-05-01), Dixon et al.
patent: 4042329 (1977-08-01), Hochstrasser
patent: 4185963 (1980-01-01), Heuck
patent: 4211530 (1980-07-01), Goverde et al.
patent: 4211531 (1980-07-01), Das
Heuck et al.; Clin. Chem. 23/3, (1977); pp. 536-540.
Nishida et al.; J. Biol. Chem. 245/18, (1970); pp. 4689-4697.
Progress Colloid and Polymer Sci. 65, (1978); pp. 251-264; Dr. Dietrich Verlag.
Biochemistry, vol. 12, Nr. 14, pp. 2645-2649, (1973).

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