Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving uric acid
Patent
1983-07-22
1987-09-22
Kepplinger, Esther M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving uric acid
435 11, 435 14, 435 25, 435 28, 435810, C12Q 162, C12Q 160, C12Q 154, C12Q 126, C12Q 128
Patent
active
046955402
ABSTRACT:
Cholesterol in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with cholesterol oxidase. Similarly, a substrate such as glucose, etc., can be determined quantitatively by measuring the generated superoxide ion colorimetrically, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b') iron complex of porphyrin or ion chelate of complexane, (c) an amine and/or a phenol, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent.
REFERENCES:
patent: 4353983 (1982-10-01), Siddiqi
Halliwell et al., (1982), Interaction of the Superoxide Radical with Peroxidase and with Other Non Complexes, Oxidase Relat Redox Syst Proc Int Symp, 3rd, 1979.
Stryer, L., (1975), Biochemistry, 2nd Ed., p. 344.
Porras et al., (1981), The Reaction of Reduced Xanthine Oxidase with Oxygen, J.Biol. Chem. 256(17): 9096-9103.
Matkovics et al., (1975), Utilization of Catalase and Superoxide Dismutase to Clarify the Mechanism of Action of Oxidases, Proc. Hung. Annu. Meet. Biochem. 15:95, Chem. Abstr. 88:46907w.
Galliani et al., (1980), Formation of Superoxide Radical Anion in the Horseradish Peroxides-Catalyzed Oxidation of three Aromatic Tertiary Amines with Hydrogen Peroxide, J. Chem. Soc. (1) 1-3, Chem. Abstr. 92:18038f.
Hanada Toshiro
Yamanishi Kazuhiko
Kepplinger Esther M.
Wako Pure Chemical Industries Ltd.
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