Quantitative determination of pyruvic acid and quantitative anal

Details Quantitative determination of pyruvic acid and quantitative anal Quantitative determination of pyruvic acid and quantitative anal

1993-09-02

1995-09-05

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 4, 435 74, 435 791, 435 25, C12Q 100, C12Q 132

Patent

active

054478472

ABSTRACT:
Disclosed herein is a quantitative determination of pyruvic acid, which comprises reacting oxidized nicotinamide adenine dinucleotide, a pyruvate dehydrogenase complex and coenzyme A to a specimen and measuring the amount of the resulting reduced nicotinamide adenine dinucleotide. A quantitative analysis for a component of a living body, in which the quantitative determination is used, is also disclosed. Pyruvic acid or other components existing in a living body or formed in the course of a reaction in the living body can be quantitatively determined simply and precisely.

REFERENCES:
patent: 4923796 (1990-05-01), Deneke
Sigma Catalog, 1993, (published 1992), p. 866.
Methods in Enzymology, vol. 89, (1982), pp. 35-40.
Biochemical Medicine, vol. 29, pp. 51-56 (1983).
Rupert et al., Tetrahedron Letters, vol. 28, No. 52, pp. 6583-6586 (1987).
Singleton et al., Dictionary of Microbiology and Molecular Biology, (1987), (p. 978).

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