Chemistry: analytical and immunological testing – Lipids – triglycerides – cholesterol – or lipoproteins
Patent
1992-09-09
1996-02-13
Redding, David A.
Chemistry: analytical and immunological testing
Lipids, triglycerides, cholesterol, or lipoproteins
436164, 436166, G01N 3392
Patent
active
054910935
ABSTRACT:
Lipid is determined quantitatively by treating a sample with a surfactant in an aqueous medium to obtain a liquid sample dispersion, then reacting the dispersion with a reagent containing cupric ion and bicinchoninic acid, and measuring a colored state developed by complex formation of bicinchoninic acid with cuprous ion formed from cupric ion in the presence of lipid. This method can be effectively applied to determination of amphiphilic lipid having a tendency of gathering in an aqueous medium. Further, two different species interfering with each other in quantitative determination thereof are determined respectively by conducting two different determination methods on the same sample followed by calculation using the values obtained.
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Kawaguchi Masahiro
Nomoto Tsuyoshi
Ohyama Junji
Tomida Yasuko
Yamamoto Nobuko
Canon Kabushiki Kaisha
Redding David A.
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