Quantitative determination of L-fucose

Details Quantitative determination of L-fucose Quantitative determination of L-fucose

1987-01-14

1990-05-22

Deck, Randall E.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 18, 435190, 435843, C12Q 132, C12Q 134, C12N 904, C12R 115

Patent

active

049277537

ABSTRACT:
A method is provided for the quantitative determination of L-fucose in a sample solution. According to the method an L-fucose dehydrogenase having its optimum pH around neutrality is allowed to act upon the sample solution in the presence or absence of an .alpha.-L-fucosidase, and the amount of reduced nicotinamide adenine dinucleotide thus formed is measured.

REFERENCES:
Schachter et al., Journal of Biological Chemistry, vol. 244, No. 17, Sep. 10, 1969, pp. 4785-4792.
Barman, Enzyme Handbook, Supplement I, Springer-Verlag, New York, (1974) p. 48.

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