Quantitative determination of glucose utilizing enzyme cascade s

Details Quantitative determination of glucose utilizing enzyme cascade s Quantitative determination of glucose utilizing enzyme cascade s

1989-09-25

1993-05-25

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 4, 435 5, 435 15, 435 18, 435 25, 435 26, 436 68, 436 95, 436164, 436815, C12Q 154

Patent

active

052139662

ABSTRACT:
A procedure is disclosed for the determination of glucose in a biological liquid, as well as a reagent mixture for use in conjunction with the procedure. The biological liquids concerned are especially blood, blood components, urine and spinal fluid. The procedure is based on a two-phase enzymatic reaction, the first phase of which comprises transforming glucose with the aid of ATP (adenosine-5-triphosphate) and hexokinase into glucose-6-phosphate and the second phase of which comprises transforming glucose-6-phosphate with the aid of NAD and glucose-6-phosphate dehydrogenase into 6-phosphogluconate. ATP, NAD and the enzymes mentioned are included in a suitably buffered reagent mixture, which can be added as a single dose to the glucose-containing liquid sample. The reaction is monitored kinetically by measuring the absorption caused by the NADH produced in the second phase twice within approximately one minute from the initiation of the first reaction phase. The glucose content of the sample can be calculated from the measured change of absorbance.

REFERENCES:
patent: 3977944 (1976-08-01), Muller-Mallhesius et al.
patent: 4299916 (1981-11-01), Litman et al.
patent: 5037738 (1991-08-01), Lamos et al.

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