Quantitative determination of bilirubin and a reagent therefor

Details Quantitative determination of bilirubin and a reagent therefor Quantitative determination of bilirubin and a reagent therefor

1990-03-13

1992-04-14

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435 4, 435 18, 435 28, 436 97, 436903, C12Q 126, G01N 3372

Patent

active

051047942

ABSTRACT:
Disclosed is a process for measuring an amount of all bilirubins in a specimen, comprising deconjugating conjugated bilirubins with a reagent comprising an enzyme capable of deconjugation to form the unconjugated bilirubin, and determining an amount of the unconjugated bilirubin in the specimen, and a reagent therefor. The present invention also provides a process for measuring an amount of the unconjugated bilirubin in a specimen, comprising oxidizing and eliminating conjugated bilirubins in the specimen with an oxidizing agent capable of oxidizing bilirubins, in the presence of a metal ion forming a complex with the bilirubins and a surfactant at pH 6.5 or less, and determining an amount of the unconjugated bilirubin in the specimen, and a reagent therefor.

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