Quantitative detection of analytes on immunochromatographic stri

Details Quantitative detection of analytes on immunochromatographic stri Quantitative detection of analytes on immunochromatographic stri

1995-01-30

1996-10-29

Housel, James C.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436523, 436525, 436513, 436514, 436810, 435810, 435805, 435970, 422 56, G01N 33544

Patent

active

055696082

ABSTRACT:
A method for determining the concentration of analyte in a test fluid by immunochromatography techniques which involves quantitatively determining the signals from captured analyte/labeled binding partner complex by an instrument, e.g. reflectance spectrometer. In a preferred embodiment, a reflectance reading is determined for the captured complex and uncomplexed labeled binding partner which is captured in a separate zone of the immunochromatographic strip and the ratio of these reflectances is used to provide additional quantitation to the assay method.

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