Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1980-02-13
1981-11-17
Hruskoci, Peter A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435 25, 435 27, 435 28, 435189, 435810, 435923, 435924, C12Q 100, C12Q 126
Patent
active
043012442
ABSTRACT:
There is provided a method for the quantitative analysis of a free fatty acid which comprises (1) the first step of treating a sample containing the free fatty acid with acyl-coenzyme A synthetase in the presence of adenosine triphosphate and coenzyme A to form acyl-coenzyme A, (2) the second step of oxidizing said acyl-coenzyme A, in the presence of oxygen, with acyl-coenzyme A oxidase produced by a microorganism of the genus Candida to form enoyl-coenzyme A and hydrogen peroxide, and (3) the third step of (a) measuring the amount of the formed enoyl-coenzyme A or hydrogen peroxide or (b) measuring the amount of oxygen consumed in said oxidation reaction, to thereby determine the amount of free fatty acid in said sample.
REFERENCES:
patent: 3979262 (1976-09-01), Hunziker
patent: 4071413 (1978-01-01), Takahashi et al.
patent: 4101381 (1978-07-01), Klose et al.
patent: 4229538 (1980-10-01), Yamada et al.
Chemical Abstracts, 89:159269d, "Acyl-CoA Oxidase of Rat Liver", p. 220, 1978.
Chemical Abstracts, 85:188245r, A Soluble-Coenzyme A Oxidase from the Yeast Candida utilis, 1976.
Chemical Abstracts, 90:135464h, Subcellular Distribution of the Enzymes of Fatty Acyl-CoA B-Oxidation System, 1979.
Takahashi et al. "Rinsho Kagaku", 4, 1975, pp. 179-185.
Ando Makoto
Kikuchi Toshiro
Hruskoci Peter A.
Toyo Boseki Kabushiki Kaisha
LandOfFree
Quantitative analysis of free fatty acid and reagent composition does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Quantitative analysis of free fatty acid and reagent composition, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Quantitative analysis of free fatty acid and reagent composition will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1293231