Quantitative analysis of DNA

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C536S022100, C536S124000

Reexamination Certificate

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11138981

ABSTRACT:
The present invention is related to a novel approach for quantitation of DNA based on the stoichiometric existence of phosphorus (P) in DNA.

REFERENCES:
Baginski et al., Microdetermination of nucleiic acid phosphate. Microchemical J. 13 : 115-119 (1968).
Kirkpatrick et al., Simplified wet ash procedure for total phosphorus analysis of organophosphates in biological samples. Analytical Chemistry 43(12) :1707-1709 (1971).
Fiske et al, The colorimetric determination of phosphorus. J. of Biological Chemistry 66:375-400(1925).
Chen et al., Microdetermination of phosphorus 28: 1756-1758 (1956).
“MALDI on a Chip: Analysis of Arrays of Low-Femtomole to Subfemtomole Quantities of Synthetic Olignucleotides and DNA Diagnostic Products Dispensed by a Piezoeletric Pipet”; Authors: Daniel P. Little, et al.; Anal. Chem., vol. 69, No. 22; Nov. 15, 1997; pp. 4540-4546.
“Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Methods for Oligodeoxynucleotides: Improvements in Matrix, Detection Limits, Quantification, and Sequencing”; Authors: Li-Kang Zhang and Michael L. Gross; American Society for Mass Spectrometry; 2000; pp. 854-865.
“Quantitation of Oligonucleotides by Phosphodiestrerase Digestion Followed by Isotope Dilution Mass Spectrometry: Proof of Concept”; Authors: Gavin O'Connor, et al.; Anal. Chem., vol. 74, No. 15; Aug. 1, 2002; pp. 3670-3676.
“Revised UV extinction coefficients for necleoside-5′-monophosphates and unpaired DNA and RNA”; Authors: Michael J. Cavaluzzi and Philip N. Borer; Nucleic Acids Research, vol. 32, No. 1; 2004; pp. 1-9.
“A Comparison of Measured and Calculated Single- and Double -Stranded Oligodeoxynucleotide Extinction Coefficients”; Authors: Gary Kallansrud and Brian Ward; Analytical Biochemistry, vol. 236, Article No. 0141; 1996; pp. 134-138.
Techanical Brief—“Quantifying DNA concentrations using fluorometry: A comparison of fluorophores”; Authors: Kalpana Rengarajan, et al.; Molecular Vision, vol. 8; 2002; pp. 416-421.
“Characteristics of Different Nucleic Acid Staining Dyes for DNA Fragment Sizing by Flow Cytometry”; Authors: Xiamei Yan, et al.; Anal. Chem., vol. 71, 1999, pp. 5470-5480.
“Concentration and Extinction Coefficient Determination for Oligonucleotides and Analogs Using a General Phosphate Analysis”; Authors: James H. Murphy and Tina L. Trapane; Analytical Biochemistry, vol. 240, Article No. 0357; 1996; pp. 273-282.

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