Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1986-05-12
1989-03-28
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435 25, C12Q 132
Patent
active
048163945
ABSTRACT:
A 3.alpha.-hydroxysteroid contained in a sample can be quantitatively analyzed by causing a 3.alpha.-hydroxysteroid dehydrogenase to act on the sample in the presence of nicotinamide adenine dinucleotide, causing tetrazolium or a salt thereof, an electron carrier substance, and 3-oxosteroid-.DELTA..sup.1 -dihydrogenase and/or 3-oxosteroid-.DELTA..sup.4 -dehydrogenase to act on the thus-obtained reaction product, and then quantitatively analyzing the resultant formazan.
REFERENCES:
Papanastasiou-Diamandi-Chem. Abst., vol. 101 (1984) p. 106700x.
Papanastasiou-Diamandi-Chem. Abst., vol. 99 (1983) p. 209041c.
"Comprehensive Biochemistry", vol. 13, 3rd Edition, pp. 80, 81, edited by M. Florkin et al., Elsevier Scientific Publishing Co., Amsterdam, NL; *p. 8, lines 6-11; p. 81, lines 13-18.
Fresenius Zeitschrift fur Analytische Chemie, vol. 290, 1978, pp. 181, 182, Springer-Verlag, Berlin, DE; C. C. Gillhuus-Moe et al.: "Methodological Studies on a Direct Enzymatic Fluorometric Quantitation of Total Bile Acids in Serum".
Akimoto Toshiyuki
Hirata Miyoshi
Maki Akemichi
Nagasaki Senkichi
Ushizawa Koji
Daiichi Pure Chemicals Co. Ltd.
Rosen Sam
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