Quantitation of nucleic acids using the polymerase chain reactio

Details Quantitation of nucleic acids using the polymerase chain reactio Quantitation of nucleic acids using the polymerase chain reactio

1989-09-28

1993-06-15

Moskowitz, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 536 2433, 935 77, 935 78, C12Q 168, C12P 1934, C12N 1500, C07H 2104

Patent

active

052197271

ABSTRACT:
The present invention provides a method for determining the amount of a target acid segment in a sample by polymerase chain reaction. The method involves the simultaneous amplification of the target nucleic acid segment and an internal standard nucleic acid segment. The amount of amplified DNA from each segment is determined and compared to standard curves to determine the amount of the target nucleic acid segment present in the sample prior to amplification. The method is especially preferred for determining the quantity of a specific mRNA species in a biological sample. Additionally, an internal standard is provided useful for quantitation of multiple mRNA species.

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