Quantification of hydroperoxides using prostaglandin H synthase

Details Quantification of hydroperoxides using prostaglandin H synthase Quantification of hydroperoxides using prostaglandin H synthase

1984-07-09

1987-06-23

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 25, 435 28, 435184, C12Q 168, C12Q 126, C12Q 128, C12N 999

Patent

active

046752811

ABSTRACT:
A method of assessing certain disease conditions of body tissues and body fluids by the measurement of hydroperoxides therein; includes using the enzyme prostaglandin H synthase and observing the rate of enzyme-catalyzed oxygenation reaction, preferably with equipment such as a polarographic oxygen electrode that monitors the oxygen concentration. The presence of increased amounts of hydroperoxide cause a faster attainment of the optimal rate of enzyme-catalyzed oxygen consumption. This enzymic assay provides high sensitivity and selectivity for hydroperoxides. Cyanide may also be introduced to temper the reaction velocity to facilitate monitoring the enzyme reaction.

REFERENCES:
patent: 3769172 (1973-10-01), Bressler et al.
patent: 4368261 (1983-01-01), Klose et al.
patent: 4368262 (1983-01-01), Bucovaz et al.
Yagi, Biochem. Med., 15, 212-216 (1976).
Perez, et al., Inflammation 4, 313-328 (1980).
Sato, et al., Biochem. Med., 21, 104-107 (1979).
Smith, et al., Biochem. Pharmacol., 31, 19-26 (1982).
Nishigaki, et al., Biochem. Med., 25, 373-378 (1981).
Nishigaki, et al., Biochem. Med., 24, 185-189 (1980).
Ottolenghi, et al., Arch. Biochem. Biophys., 79, 355-363 (1959).
Howes, et al., Resh. Comm. Chem. Path. Pharmacol., 2, 619-625 (1971).
Kulmacz, et al., Prostaglandins, 25, 531-540 (1983).
Lieberman et al., Science, 152, 213-214 (1966).
Dillard, et al., Lipids, 14, 989-995 (1979).
Graff, Meth. in Enzymology, 86, 386-392 (1982).
Hemler et al., J. Biol. Chem., 255, 6253-6261 (1980).
Asakawa, et al., Lipids, 15, 137-140 (1980).
Barber et al., Adv. Gerontol. Resh., 2, 355-397 (1967).
Bridges, et al., Ann. N.Y. Acad. Sci., 407, 42-63 (1983).

Affiliated with

Also associated with

Rating

Quantification of hydroperoxides using prostaglandin H synthase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Quantification of hydroperoxides using prostaglandin H synthase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Quantification of hydroperoxides using prostaglandin H synthase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-720934