Quantification of fibrinogen in whole blood samples contained in

Chemistry: analytical and immunological testing – Clotting or clotting factor level tests

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73 611R, 73 614, 73 641, 210782, 436 70, 73 6143, 73 6163, 73 6141, G01N 3102, G01N 3386

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051378329

ABSTRACT:
Quantification of blood plasma fibrinogen content is made in a sample of anticoagulated whole blood contained in a sampling tube. The tube is a transparent centrifuge tube which contains a sample constituent layer-elongating float. The blood sample is centrifuged in the tube containing the float, and various cell constituent measurements are made. The centrifuged sample is then heated and recentrifuged to cause the precipitated fibrinogen to layer out on top of the float remote from the buffy coat band in the plasma layer. The thickness of the fibrinogen band is then measured, whereby quantification of the fibrinogen content of the blood sample can be made by a precalibrated instrument.

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Millar et al., Chemical Abstracts, vol. 76, No. 23, Abstract No. 76:137699y, 1971.

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