Quantification of bacteria using a nucleic acid hybridization as

Details Quantification of bacteria using a nucleic acid hybridization as Quantification of bacteria using a nucleic acid hybridization as

1993-04-01

1994-08-02

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 2432, 935 77, 935 78, C12Q 168, C07H 2104, C07H 2102, C12N 1500

Patent

active

053345010

ABSTRACT:
This invention provides for a method of quantifying bacteria using a bacterial specific nucleic acid probe which is complementary to a unique and highly conserved region of the 16S ribosomal RNA (rRNA) of bacteria. This probe permits the rapid detection of 16S rRNA in a sample and by comparison with known standards, one can estimate the total bacterial count in the sample. The method is accurate and reproducible and conducted at temperatures of between about 120.degree. to about 40.degree. C.

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