Pyruvate oxidase mutants, DNA expressing pyruvate oxidase and me

Details Pyruvate oxidase mutants, DNA expressing pyruvate oxidase and me Pyruvate oxidase mutants, DNA expressing pyruvate oxidase and me

1991-03-14

1992-03-17

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435189, C12N 902

Patent

active

050968211

ABSTRACT:
The present invention provides a pyruvate oxidase which decarboxylates pyruvate to form inter alia hydrogen peroxide and is active without the addition of FAD, thiamine pyrophosphate and divalent metal ions. The amino acid sequence of the enzyme changes at least one proline in position 178 and alanine in position 458 to a different amino acid. The present invention also provides a process for the preparation of this pyruvate oxidase and methods of use thereof.

REFERENCES:
patent: 4666832 (1987-05-01), Elstner et al.

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