Pyruvate orthophosphate dikinase gene, recombinant DNA, and proc

Details Pyruvate orthophosphate dikinase gene, recombinant DNA, and proc Pyruvate orthophosphate dikinase gene, recombinant DNA, and proc

1997-10-01

2000-04-25

Prouty, Rebecca E.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

536 231, 536 232, 435 712, 4352523, 43525233, 4353201, C12N 912, C12N 1554, C12N 1563, C12N 121

Patent

active

060543051

ABSTRACT:
Disclosed are a pyruvate orthophosphate dikinase gene coding for a polypeptide containing the amino acid sequence of SEQ ID NO:1 or an amino acid sequence where in said amino acid sequence one or more amino acids are added, deleted or substituted and bringing about the activity of pyruvate orthophosphate dikinase; recombinant DNA having the pyruvate orthophosphate dikinase gene integrated in vector DNA; and a process for producing pyruvate orthophosphate dikinase, which comprises culturing a microorganism belonging to the genus Escherichia carrying the above recombinant DNA in a medium, and recovering pyruvate orthophosphate dikinase from the resulting culture. The pyruvate orthophosphate dikinase gene and a process for producing pyruvate orthophosphate dikinase are provided.

REFERENCES:
E. Saavedra-Lira et al., "Cloning and Sequence Determination of the Gene Coding for the Pyruvate Phosphate Dikinase of Entamoeba histolytica", Gene 142(2): 249-251, 1994.
L. Nevalainen et al., "Sequence of a Giardia lamblia Gene Coding for the Glycolytic Enayme, Pyruvate Phosphate Dikinase", Mol. Biochem. Parasitol. 77(2): 217-223, 1996.
Kikkoman, "Pyruvate-Orthophosphate-Dikinase and Production Thereof", Derwent-Biotechnol. Absts. 15(21): 104, ABst. No. 96-12552 Jul. 1996.
Sakakibara et al., 116th Annual Meeting of Pharmaceutical Society of Japan, Lecture Summary 4, p. 154 (1996).
Hoffman et al., "A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli," Gene 57: 267-272 (1987).
Morrison, "Transformation and Preservation of Competent Bacterial Cells by Freezing," Methods in Enzymology 68:326-331 (1979).
Hohn, "In Vitro Packaging of .lambda. and Cosmid DNA," Methods in Enzymology 68: 299-309 (1979).
Messing, "New M13 Vectors for Cloning," Methods in Enzymology 101: 20-78 (1983).
Niersbach et al., "Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase," Mol. Gen. Genet. 2341: 332-336 (1992).

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