Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2005-04-26
2005-04-26
Monshipouri, Maryam (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S183000
Reexamination Certificate
active
06884606
ABSTRACT:
The present invention concerns an anaplerotic enzyme fromCorynebacterium glutamicumwhich replenishes oxaloacetate consumed during lysine and glutamic acid production in industrial fermentations. In particular, isolated nucleic acid molecules are provided encoding the pyruvate carboxylase protein. Pyruvate carboxylase polypeptides are also provided.
REFERENCES:
patent: 4275157 (1981-06-01), Tosaka et al.
patent: 6171833 (2001-01-01), Sinskey et al.
patent: 0 723 011 (1996-07-01), None
patent: WO 9918228 (1999-04-01), None
Altschul, S.F. et al., “Basic Local Alignment Search Tool,”J. Mol. Biol. 215:403-410, Academic Press, Inc., New York, NY (1990).
Attwood, P.V., “The Structure and the Mechanism of Action of Pyruvate Carboxylase,”Int. J. Biochem. Cell Biol. 27:231-249, Pergamon Press, Exeter, England (1995).
Brewster, N.K. et al., “Regulation of Pyruvate Carboxylase Isozyme (PYC1, PYC2) Gene Expression inSaccharomyces cerevisiaeduring Fermentative and Nonfermentative Growth,”Arch. Biochem. Biophys. 311:62-71, Academic Press, New York, NY (1994).
Charles, A.M. and Willer, D.W. “Pyruvate carboxylase fromThiobacillus novellus: properties and possible function,”Can. J. Microbiol. 30:532-539, National Research Council Of Canada, Ottawa, Canada (1984).
Dunn, M.F. et al., “Pyruvate Carboxylase fromRhizobium etli: Mutant Characterization, Nucleotide Sequence, and Physiological Role,”J. Bacteriol. 178:5960-5970, American Society for Microbiology, Baltimore, MD (1996).
Fry, D.C. et al., “ATP-binding site of adenylate kinase: Mechanistic implications of its homology with ras-encoded p21, F1-ATPase, and other nucleotide-binding proteins,”Proc. Natl. Acad. Sci. USA 83:907-911, National Academy of Sciences of the USA, Washington, D.C. (1986).
Gubler, M. et al., “Effects of phosphoenol pyruvate carboxylase deficiency on metabolism and lysine production inCorynebacterium glutamicum,” Appl. Microbiol. Biotechnol. 40:857-863, Springer-Verlag, Berlin, Germany (1994).
Hanahan, D., “Studies on Transformation ofEscherichia coliwith Plasmids,”J. Mol. Biol. 166:557-580, Academic Press, Inc., New York, NY (1983).
Jäger, W. et al., “ACorynebacterium glutamicumgene encoding a two-domain protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins,”Arch. Microbiol. 166:76-82, Springer-Verlag, Berlin, Germany (1996).
Jetten, M.S.M. and Sinskey, A.J., “Characterization of phosphoenolpyruvate carboxykinkase fromCorynebacterium glutamicum,” FEMS Microbiol. Lett. 111:183-188, Elsevier/North Holland, Amsterdam (1993).
Keilhauer, C. et al., “Isoleucine Synthesis inCorynebacterium glutamicum: Molecular Analysis of theilvB-ilvN-ilvCOperon,”J. Bacteriol. 175:5595-5603, American Society for Microbiology, Baltimore, MD (1993).
Koffas, M.A.G. et al., “Sequence of theCorynebacterium glutamicumpyruvate carboxylase gene,”Appl. Microbiol. Biotechnol. 50:346-352, Springer-Verlag, Berlin, Germany (Sep. 1998).
Koffas, M.A.G., et al., GenEmbl database, Accession No. AF038548, alignment results (Sep. 1998).
Kondo, H. et al., “Cloning and nucleotide sequence ofBacillus stearothermophiluspyruvate carboxylase,”Gene 191:47-50, Elsevier Science Publishers B.V., Amsterdam, Netherlands (May 1997).
Kumar, G.K. et al., “Involvement and Identification of Tryptophanyl Residue at the Pyruvate Binding Site of Transcarboxylase,”Biochem. 27:5978-5983, American Chemical Society, Washington D.C. (1988).
Lim, F et al., “Sequence and Domain Structure of Yeast Pyruvate Carboxylase,”J. Biol. Chem. 263:11493-11497, American Society for Biochemistry and Molecular Biology, Inc., Baltimore, MD (1988).
Milrad de Forchetti, S.R. and Cazzulo, J.J., “Some Properties of the Pyruvate Carboxylase fromPseudomonas fluorescens,” J. Gen. Microbiol. 93:75-81, Cambridge University Press, Cambridge, England (1976).
Momose, H. et al., “On the Transducing Phages in Glutamic Acid-Producing Bacteria,”J. Gen. Appl. Microbiol. 22:119-129, Microbiology Research Foundation, Tokyo, Japan (1976).
Mukhopadhyay, B. et al., “Purification, Regulation, and Molecular and Biochemical Characterization of Pyruvate Carboxylase fromMethanobacterium thermoautotrophicumStrain ΔH,”J. Biol. Chem. 273:5155-5166, American Society for Biochemistry and Molecular Biology, Inc., Baltimore, MD (Feb. 1998).
O'Brien, R.W. et al., “Novel Enzymic Machinery for the Metabolism of Oxalacetate, Phosphoenolpyruvate, and Pyruvate inPseudomonas citronellolis,” J. Biol. Chem. 252:1257-1263, American Society for Biochemistry and Molecular Biology, Inc., Baltimore, MD (1977).
Park, S.M. et al., “Elucidation of anaplerotic pathways inCorynebacterium glutamicumvia13C-NMR spectroscopy and GC-MS,”Appl. Microbiol. Biotechnol. 47:430-440, Springer-Verlag, Berlin, Germany (Apr. 1997).
Peters-Wendisch, P.G. et al., “Phosphoenolpyruvate carboxylase inCorynebacterium glutamicumis dispensable for growth and lysine production,”FEMS Microbiol. Lett. 112:269-274, Elsevier/North Holland, Amsterdam, Netherlands (1993).
Peters-Wendisch, P.G. et al., “C3-Carboxylation as an anaplerotic reaction in phosphoenolpyruvate carboxylase-deficientCornybacterium glutamicum,” Arch. Microbiol. 165:387-396, Springer-Verlag, Berlin, Germany (1996).
Peters-Wendisch, P.G. et al., “Pyruvate carboxylase as an anaplerotic enzyme inCornybacterium glutamicum,” Microbiology 143:1095-1103, Kluwer Academic/Plenum Publishers, Washington, D.C. (Apr. 1997).
Peters-Wendisch, P.G. et al., “Pyruvate carboxylase fromCorynebacterium glutamicum: characterization, expression and inactivation of the pyc gene,”Microbiology 144:915-927, Kluwer Academic/Plenum Publishers, Washington, D.C. (Apr. 1998).
Post, L.E. et al., “Dissection of the Functional Domains ofEscherichia coliCarbamoyl Phosphate Synthetase by Site-directed Mutagenesis,”J. Biol. Chem. 265:7742-7747, American Society for Biochemistry and Molecular Biology, Inc., Baltimore, MD (1990).
Reyes, O. et al., “‘Integron’-bearing vectors: a method suitable for stable chromosomal integration in highly restrictive Corynebacteria,”Gene 107:61-68, Elsevier Science Publishers B.V., Amsterdam, Netherlands (1991).
Scrutton, M.C. and Taylor, B.L., “Isolation and Characterization of Pyruvate Carboxylase fromAzotobacter vinelandiiOP,”Arch. Biochem. Biophys. 164:641-654, Academic Press, New York, NY (1974).
Serwold-Davis, T.M. et al., “Transformation ofCornyebacterium diphtheriae, Corynebacterium ulcerans, Cornyebacterium glutamicum, andEscherichia coliwith theC. diphtheriaeplasmid pNG2,”Proc. Natl. Acad. Sci. USA 84:4964-4968, National Academy of Sciences of the USA, Washington, D.C. (1987).
Simon, R. et al., “A Broad Host Range Mobilization System For In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria,”Bio/Technol. 1:784-791, Nature Publishing Co., New York (1983).
Sonnen, H. et al., “Characterization of pGA1, a new plasmid fromCornyebacterium glutamicumLP-6,”Gene 107:69-74, Elsevier Science Publishers B.V., Amsterdam, Netherlands (1991).
Toh, H. et al., “Molecular evolution of phosphoenolpyruvate carboxylase,”Plant, Cell and Environ. 17:31-43, Blackwell Scientific Publications, New York, NY (1994).
Tomioka, N. et al., “Molecular Cloning and Characterization of Ribosomal RNA Genes from a Blue-Green Alga,Anacystis nidulans,” Mol. Gen. Genet. 184:359-363, Springer-Verlag, New York, NY (1981).
Tosaka, O. et al., “The Role of Biotin-Dependent Pyruvate Carboxylase in L-Lysine Production,”Agric. Biol. Chem. 43(7):1513-1519, Agricultural Chemical Society of Japan, Tokyo, Japan (1979).
Towbin, H. et al., “Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedur
Lessard Philip A.
Sinskey Anthony J.
Willis Laura B.
Buchanan & Ingersoll PC
Massachusetts Institute of Technology
Monshipouri Maryam
LandOfFree
Pyruvate carboxylase from Corynebacterium glutamicum does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Pyruvate carboxylase from Corynebacterium glutamicum, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Pyruvate carboxylase from Corynebacterium glutamicum will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3420508