Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1998-02-24
2000-09-12
Nashed, Nashaat
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
4352523, 4353201, 435189, 435232, 536 231, 536 232, 536 237, F04B 2203
Patent
active
061176702
ABSTRACT:
The present invention is directed to the production of pyrrolnitrin in a host via recombinant expression of the polypeptides needed to biologically synthesize pyrrolnitrin. Genes isolated from P. fluorescens, P. pyrrocinia, B. cepacia, and M. fulvus that encode polypeptides necessary to produce pyrrolnitrin are provided, along with methods for identifying and isolating genes needed to recombinantly biosynthesize pyrrolnitrin from any organism capable of producing pyrrolnitrin. The isolated genes may be transformed and expressed in a desired host organisms to produce pyrrolnitrin according to the invention for a variety of purposes, including protecting the host from a pathogen, developing the host as a biocontrol agent, and producing large, uniform amounts of pyrrolnitrin.
REFERENCES:
patent: 4306027 (1981-12-01), Alexander et al.
patent: 4456684 (1984-06-01), Weller et al.
patent: 4695455 (1987-09-01), Barnes et al.
patent: 4729951 (1988-03-01), Ferenczy et al.
patent: 4798723 (1989-01-01), Dart et al.
patent: 4812512 (1989-03-01), Bernestein et al.
patent: 4880745 (1989-11-01), Kijima et al.
patent: 4940840 (1990-07-01), Suslow et al.
patent: 4948413 (1990-08-01), Maekawa et al.
patent: 4952496 (1990-08-01), Studier et al.
patent: 4970147 (1990-11-01), Huala et al.
patent: 4975277 (1990-12-01), Janisiewicz et al.
patent: 4994495 (1991-02-01), Clough et al.
patent: 4999381 (1991-03-01), Crowley et al.
patent: 5008276 (1991-04-01), Clough et al.
patent: 5041290 (1991-08-01), Gindrat et al.
patent: 5049379 (1991-09-01), Handelsmon et al.
patent: 5059605 (1991-10-01), Clough et al.
patent: 5068105 (1991-11-01), Lewis et al.
patent: 5279951 (1994-01-01), Terasawa et al.
Albright et al., Annu. Rev. Genet., 23:311-336 (1989).
Baker et al., Biological Control of Plant Pathogens, 61-106 (American Phytopathological Society, St. Paul, Minn. 1982).
Bourret et al., Annu. Rev. Biochem., 60:401-441 (1991).
Brisbane et al., Antimicrobiol. Agents and Chemotherapy, 31(12):1967-1971 (1987).
Brisbane et al., Soil Biol. Biochem., 21(8):1019-1026 (1989).
Chen et al., "Cloning and Expression of a DNA Sequence Conferring Cephamycin C Production", Biotechnology, 6(10):1222-1224 (1988).
Clarke et al., J. Bacteriol. 154:508-512 (1983).
Cook et al., Soil Biol. Biochem., 8:269-273 (1976).
Ding et al., Gene, 33(3):212-321 (1985).
Ditta et al., PNAS:USA, 77:7347-7351 (1980).
Gaffney et al., "Global Regulation of Expression of Antifungal Factors by Pseudomonas fluorescens biological Control Strain", Molecular Plant-Microbe Interactions, 7(4):455-463 (1994).
Gambello et al., J. Bacteriology, 173(9): 3000-3009 (1991).
Gurusiddaiah et al., Antimicrobiol. Agents and Chemotherapy, 79(3): 488-495 (1986).
Gutterson et al., Journal of Bacteriology, 165(3):696-703 (1986).
Hain et al., "Disease resistance results from foreign phytoalexin expression in a novel plant", Nature, 361:153-156 (1993).
Hamden et al., Applied and Environ. Microbiol. 57:3270-3277 (1991).
Horn et al., J. Bacteriology, 170(10):4699-4705(1988).
Howell et al., Can. J. Microbiol., 29:321-324 (1983).
Howell et al., Phytopathology, 69(5):480-482 (1979).
Howell et al., Phytopathology, 69:480-482 (1979).
Howell et al., Phytopathology, 70:712-715 (1980).
Howie et al., Phytopathology, 79(10):1160 (1989).
Inouye et al., J. Bacteriology, 155(3):1192-1199 (1983).
James et al., Applied and Environmental Microbiology, 52(5):1183-1189 (1986).
Jayaswal et al., Can. J. Microbiol., 38(4):309-312 (1992).
Jeenes et al., Mol. Gen. Genet., 203:421-429 (1986).
Kaphammer et al., J. Bacteriology, 172(10):5856-5862 (1990).
Keel et al., Symbiosis, 9(1-3):327-341 (1990).
Kirner et al., Microbiology, 142:2129-2135 (1996).
Klee et al., The Plant Cell, 3:1187-1193 (1991).
Kloepper et al., Phytopathology, 71:1020-1024 (1981).
Kraus et al., Phytopathology, 79(8):910 (1989).
Kroos et al., PNAS USA, 81:5816-5820 (1984).
Lam et al., "Genetic regulation of biocontrol factors in Pseudomonas fluorescens", Third International Workshop on Plant-Promoting Rhizobacteria, Australia 97-99 (1994).
Laville et al., PNAS USA, 89:1562-1566 (1992).
Lievens et al., Pesticide Science, 27(2):141-154 (1989).
Loper, Phytopathology, 78:166-171 (1988).
Maruzen Oil Abstract, 27 (1979).
Mekalanos, J.J., J. Bacteriology, 174:1-7 (1992).
Mermod et al., J. Bacteriology, 167(2):447-454 (1986).
Mohr et al., Molecular Microbiology, 4(12):2103-2110 (1990).
Moolenaar et al., Nucl. Acids Res., 15(10):4273-4289 (1989).
Orlik-Eisel et al., Microbiology, 153(6): 561-568 (1990).
Pfender et al., Phytopathology, 83:1223-1228 (1993).
Ramos et al., Science, 235(4788):593-596 (1987).
Rothmel et al., J. Bacteriology, 173(15):4717-4724(1991).
Schell, M.A., Gene, 36(3):301-309 (1985).
Scher et al., Phytopathology, 70:412-417 (1980).
Schroth et al., Science, 216:1376-1381 (1982).
Spena et al., Mol. Gen. Genet., 227:205-212 (1991).
Starnbach et al., Molecular Microbiology, 6(4):459-469 (1992).
Stock et al., Microbiological Reviews, 53(4): 450-490 (1989).
Tanaka et al., J. Bacteriology, 170(8):3593-3600 (1988).
Thomashow et al., J. Bacteriology, 170:3499-3508 (1988).
Toder et al., Molecular Microbiology, 5(8):2003-2010 (1991).
Toohey et al., "Toxicity of Phenazine Carboxylic Acids to Some Bacteria, Algae, Higher Plants, and Animals", Canadian Journal of Botany, 43:1151-1155 (1965).
Weller et al., Journal of Cellular Biochemistry, Supplement 13A:134, Abstract CB 104 (1989).
Weller et al., Phytopathology, 73:463-469 (1983).
Wolfframm et al., FEBS Letter, 238:325-328 (1988).
"Nikkomycin-Antibiotic for Plants", NTIS TECH NOTES, 5:374 (1990) European Search Report dated Sep. 28, 1995.
Hoffmann-LaRoche & Co., "Phenazine Derivatives and a Process for the Manufacture Thereof", GB-A-1 285 010 Patent Specification, 1-8 (1972).
Weisner et al., J. Biol. Chem. 263: 13725-13732 (1988).
VanPee, K-H., Biotech. Adv. 8:185-205 (1990).
Hammer Philip Eugene
Hill Dwight Steven
Kirner Sabine
Lam Stephen Ting
Ligon James Madison
Meigs J. Timothy
Nashed Nashaat
Novartis Finance Corporation
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