Pyrophosphate-based method and apparatus for sequencing nucleic

Details Pyrophosphate-based method and apparatus for sequencing nucleic Pyrophosphate-based method and apparatus for sequencing nucleic

1988-03-25

1990-11-20

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 8, C12Q 168, C12Q 166

Patent

active

049719033

ABSTRACT:
A method for sequencing nucleic acid polymers is provided in which the polymer to be sequenced acts as a template for the production of a complementary polymer by a polymerase enzyme. The template polymer is introduced into a polymerization environment in which production of the complementary polymer will occur if appropriate nucleotides are provided. The nucleotides are then provided to the polymerization environment one at a time in individual feedstocks. If the nucleotide in a feedstock is complementary to the next base in the template polymer, i.e., the unpaired base closest to the growing end of the complementary polymer, polymerization will occur lengthening the complementary polymer and releasing PPi. By separately recovering each feedstock and analyzing it for the presence of PPi, the sequence of the complementary polymer and thus the template polymer is determined.

REFERENCES:
Sanger et al, Proc Natl Acad Sci 74(12) (1977), pp. 5463-5467.
Maxam et al, Proc Natl Acad Sci 74(2) (1977), pp. 560-564.
Wu et al., J. Molecular Biology 35, 523-37 (1968).
Donis-Keller, Nucleic Acid Research 8, 3133-3142 (1980).
Tabor et al., Proc. Nat'l. Acad. Sci. (U.S.A.) 84, 4767-4771 (1987).
Guranowski et al., J. Biol. Chem. 261, 5943-5946 (1986).
Idahl et al., Analytical Biochem. 155, 177-181 (1986).
Ugarova et al., Analytical Biochem. 158, 1-5 (1986).
DeWet et al., Proc. Nat'l. Acad. Sci. (U.S.A.) 82, 7870-7873 (1985).
Kricka et al., Analytical Biochem. 129, 392-397 (1983).
Moyer et al., Analytical Biochem. 131, 187-189 (1983).
Lundin, in Luminescent Assays: Perspectives in Endocrinology and Clinical Chemistry (Send and Pazzagli, eds) 1, 29-45 (1982).
Nicholas et al., Analytical Biochem. 114, 396-397 (1981).
Brovko et al., Biokhimiya 45, 1582-1588 (1980).
Brovko et al., Biokhimiya 43, 798-805 (1978).
Lee et al., Analytical Biochem. 80, 496-501 (1977).
Lundin et al., Analytical Biochem. 75, 611-620 (1976).
DeLuca et al., Biochemistry 13, 921-925 (1974).
Balharry et al., Analytical Biochem. 40, 1-17 (1971).
McElroy et al, Arch. Biochem. Biophysics 64, 257-271 (1956).
Nyren et al., Analytical Biochem. 151, 504-509 (1985).
Robbins et al., J. Biol. Chem. 233, 686-690 (1958).
Nicholls, Biochem. J. 165. 149-155 (1965).
Berg et al., J. Biol. Chem. 210, 657-672 (1957).
Noda, in The Enzymes (Boyer, ed).
Sawhney et al., Plant Sci. Letters 6, 103-110 (1976).
Wilson et al., J. Biol. Chem. 233, 975-981 (1958).
Darrow et al., Methods in Enzymology 5, 226-235 (1962).
Seal et al., J. Biol. Chem. 251, 975-981 (1976).
Kaguni et al., Proc. Nat'l. Acad. Sci. (U.S.A.) 80, 2221-2225 (1983).
Kunkel et al., Proc. Nat'l. Acad. Sci. (U.S.A.) 78, 6734-6738 (1981).

Affiliated with

Also associated with

Rating

Pyrophosphate-based method and apparatus for sequencing nucleic does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Pyrophosphate-based method and apparatus for sequencing nucleic , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Pyrophosphate-based method and apparatus for sequencing nucleic will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-454057