Purine nucleoside phosphorylase gene therapy for human malignanc

Details Purine nucleoside phosphorylase gene therapy for human malignanc Purine nucleoside phosphorylase gene therapy for human malignanc

1993-09-14

1996-09-03

Campell, Bruce R.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4351723, 4352521, 4353201, 424450, 514 2, 514 43, 514 44, 530350, 536 232, 536 237, 536 241, 935 70, 935 72, C12N 500, C12N 1500

Patent

active

055523110

ABSTRACT:
The invention provides a method of killing replicating or non-replicating, transfected or transduced mammalian cells and bystander cells, comprising the following steps: (a) transfecting or transducing mammalian cells with a nucleic acid encoding a non-human purine nucleoside phosphorylase (PNP); and (b) contacting the transfected or transduced cells with an amount of a substrate for the purine nucleoside phosphorylase sufficient to produce a toxic purine base-analog thereby killing the transfected or transduced cells and bystander cells. In the present method of killing cells, the non-human purine nucleoside phosphorylase can be an E. coli purine nucleoside phosphorylase. The method of the invention described above can utilize a substrate that is a purine nucleoside analog. For instance, in the method provided in the Examples, the substrate is 9-(.beta.-D-2-deoxyerythropentofuranosyl)-6-methylpurine (MeP-dR).

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