Purified yeast ubiquitin hydrolase

Details Purified yeast ubiquitin hydrolase Purified yeast ubiquitin hydrolase

1988-06-24

1992-10-20

Stone, Jacqueline

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 435228, C12N 1557

Patent

active

051569680

ABSTRACT:
Ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition, which hydrolase hydrolyzes a ubiquitin-polypeptide conjugate at the amide bond linking the ubiquitin and polypeptide, thereby yielding intact polypeptide with an unconjugated, mature N-terminus. Also provided are DNA sequences encoding such ubiquitin hydrolase, as well as expression systems for its recombinant production. Processes are provided for purification of ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.

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