Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...
Patent
1992-07-23
1997-12-23
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
536 232, 435 691, 435 712, C12N 900, C12N 1552
Patent
active
057006725
ABSTRACT:
Purified thermostable DNA ligase is described that catalyzes template-dependent ligation at temperatures of about 30.degree. C. to about 80.degree. C., and which substantially retains its catalytic ability when subjected to temperatures of from about 85.degree. C. to about 100.degree. C. The thermostable DNA ligase has an estimated molecular weight of 50,000 to 70,000 daltons. A preferred thermostable DNA ligase is described that was isolated from the archaebacteria Pyrococcus furiosus. Also described are plasmid vectors for producing recombinant thermostable DNA ligase.
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Marsh Edward J.
Mathur Eric J.
Schoettlin Warren E.
Prouty Rebecca E.
Stratagene
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